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Facilities

cGMP Facility:

 

Microarray facility: AHF has procured Affymetrix (Gene Atlas) microarray facility, which will be regularly used for global gene expression as well as miRNA analysis of the patient samples.

 

This facility has

Fluidics station

Gene-strip scanner

Bioanalyzer

Dedicated server/ computer for analysis


Contact person/ Facility In-charge:



Microscopy Facility: AHF has fluorescence microscope; Olympus 1X71 attached with BD CARV II live cell imaging with Sensicam. The microscope is also attached with dedicated computer with IP Lab Software for image analysis.


Contact person/ Facility In-charge:


FACS facility: AHF had procured BD FACS Aria in the year 2008 with 3 lasers, allowing us to perfor 6 colour FACS analysis. This instrument is crucial for our day to day analysis of stem cell, .islet cell etc as well as routine analysis such as apoptosis and PI staining.

This facility has

BD FACS Aria

Dedicated computer for analysis



Contact person/ Facility In-charge:


NGS facility: AHF has ion proton (life technologies), and will be used for exome sequencing, targeted resequencing and transcriptome sequencing of the patient samples will be routinely performed.

This facility has

Ion proton sequencer

Ion chef




Contact person/ Facility In-charge:


Cell culture facility: AHF has a dedicated cell culture facility for growing human and mice transformed cell lines.


This facility has

Laminar hood

CO2 Incubator

Waterbath

Microscope

40C Friedge

-20OC Fridge

CliniMacs

Contact person/ Facility In-charge:


Histology Core: AHF has a dedicated histology section, where the research samples will be analyzed.

Contact person/ Facility In-charge:



General Biochemical Instrumentation:

Ultracentrifuge

Spectrophotometer

Electrophoresis

Western Blot

ELISA reader

Fluroscan

HPLC

RT PCR

Minor equipments (Autoclaves, water baths, pH meters, Homogenizers, Balances etc.,)

Freezers (-200c and -800C)

Millipore water system


SERVICES:

Facility based services:

NGS, Whole exome, clinical exome, targeted amplicon sequencing and transcriptome sequencing can be carried out at our facility on Ion Proton system. Interested customers can contact AHF for further details on the nature of projects and pricing. Data analysis will be performed and a list of dysregulated genes and pathways will be communicated to the customer.


Microarray: Laboratories interested in carrying out research based gene expression projects on the Gene Atlas platform can contact AHF for detailed discussion on the nature of projects and pricing information. The work includes extraction(s) of RNA, processing the sample, hybridization to the Affymetrix. Data analysis will be performed and a list of dysregulated genes and pathways will be communicated to the customer.


FACS:


AHF provides users the scientific resource assistance for high quality, multi-parameter flow cytometry analyses and sorting.

Multi-parameter flow cytometry analysis (up to 7 fluorochromes) supporting experimental protocols like

a. Immunophenotyping/immunofluorescence,

b. surface or intracellular antigens,

c. Phenotype characterization,

d. Proliferation, tracking, and cell cycle measurement (DNA content),

e. Apoptosis assays based on biochemical changes at various points in apoptotic/necrotic progression.




Clinical Lab Services:

CP Panel: Chronic pancreatitis is a long term progressive inflammatory disease of the pancreas with a genetic background. Currently we test N34S, rs17107315 (A>G) of SPINK1 gene; N29I, rs111033566 (A>T) and R122H, rs111033565 (G>A); rs10273639 of the PRSS1 gene; V235I, rs140993290 (G>A), A73T, rs515726209 (G>A) and 180C>T, rs497078 of the CTRC gene; L26V, rs12338 (C>G) and S53G, rs1803250 (A>G) of the CTSB gene; rs7057398 of CLDN-2 gene; Phe508 del, rs113993960 (-/CTT), G542X, rs113993959 (G>T), G551D, rs75527207 (G>A), R177H, rs78655421 (G>C,A,T) of the CFTR gene to assess the predisposing risk of an individual for Chronic pancreatitis.

Specimen requirement: 3ml of whole blood in EDTA vaccutainers


Gilbert Syndrome: Gilbert’s syndrome (GS) is a harmless condition in which bilirubin is not processed by the liver properly. Two polymorphisms in UGT1A1 namely G71R in exon 1(rs4148323) and A(TA)7TAA in the TATA box (rs8175347) of the promoter region are the most prevalent and are responsible for GS. Currently we test patients for the mentioned polymorphisms.

Specimen requirement: 3ml of whole blood in EDTA vaccutainers


CRC Panel: KRAS and NRAS are closely related RAS oncogene family members. The most common mutations in these genes are codons 12, 13 and 61 resulting in increased levels of guanosine triphosphate-bound RAS proteins. Identifying mutations in CRC tumor is important not only because they promote oncogenesis but also they can predict a lack of response to anti-EGFR therapy. The most common mutation in BRAF gene (V600E) has prognostic value and is found in 10% of metastatic CRC.

Specimen requirement: Formalin fixed paraffin embedded tissue blocks containing confirmed tumors are accepted for testing.


GIST Panel: Gastrointestinal stromal tumors (GIST) the common mesenchymal tumors of the gastrointestinal tract harbor a mutation affecting either the KIT or PDGFRA genes. Mutations in these genes are associated with poorer outcome and can predict can predict the response to treatment with tyrosine kinase inhibitors (Maleddu A et al. Journal of Translational Medicine. 2011;9:75.). Currently we test GIST tumors for mutations in Exons 9,11,13 and 17 of c-KIT gene and Exons 12 and 18 of PDGFRA gene.

Specimen requirement: Formalin fixed paraffin embedded tissue blocks containing confirmed tumors are accepted for testing.


NAFLD Panel: Currently we test variants namely I148M, rs738409 (C>G) of PNPLA3 gene and E167K, rs58542926 (C>T) of TM6SF2 gene to assess the risk of NAFLD in an individual. Appropriate genetic and nutritional counseling will be suggested.

Specimen requirement:


HBV/ HCV viral load: Here at AHF, we extract the viral nucleic acid from patient samples by automated method using Cobas Ampliprep(Roche Molecular Diagnostics). Quantitative Detection and estimation of viral load of these viruses will be performed using TaqMan Real time PCR assay by High Pure kit and Cobas TaqMan 48 analyzer (Roche Molecular Diagnostics). Genotyping of hepatitis C virus will be performed by probe based real time PCR assay(Roror Gene Q-Qiazen).

Specimen requirement: Serum / Plasma / Biopsy Tissue


TB: Tuberculosis: We routinely perform qualitative detection of MTB (Mycobacterium tuberculosis) by PCR based approach.

Specimen requirement: Intestinal Biopsy Tissue

TPMT: Inflammatory Bowel Disease (IBD) patients show significant upregulation of  Thiopurine Methyltransferase (TPMT) Enzyme. Here at AHF, we regularly analyze Thiopurine Methyltransferase (TPMT) Enzyme Activity in plasma samples for inflammatory Bowel Disease patients by HPLC method.

Specimen requirement: 4 ml of whole blood in Heparin / EDTA vaccutainers

 

Direction Map

Asian Healthcare Foundation
6-3-661, Somajiguda
Hyderabad Andhrapradesh
India
Pin:500082

 

Contact No
040-23378888

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